immunoblotting rabbit polyclonal anti sox2 proteintech 11064 1 ap lot  (Proteintech)

Journal: Thoracic cancer

Article Title: NSUN4 Facilitates the Activity of Oncogenic Protein CDC20 to Promote NSCLC Development by Mediating m5C Modification of CDC20 mRNA.

doi: 10.1111/1759-7714.70023

Figure Lengend Snippet: FIGURE 2 | NSUN4 inhibition affects cell growth, apoptosis, stemness, invasiveness, and migratory ability in vitro. (A–H) Various experiments were conducted using different treatment methods and cell types (A549 and SK-MES-1 NSCLC cells), with three groups: Sh-ctrl, sh-NSUN4#1, or sh-NSUN4#2. (A) Evaluation of NSUN4 protein expression by immunoblotting in cells transfected as indicated. (B) Assessment of the number of formed colonies with cells transfected as indicated. (C) Determination of cell apoptotic ratio with cells transfected as indicated by flow cytometry. (D and E) Examination of cell migratory rate and invasiveness with transfected cells. Scale bars: 100 μm. (F) Measurement of sphere formation with transfected NSCLC cells. Scale bars: 100 μm. (G and H) Expression of SOX2, CD133, and KLF4 proteins using immunoblot analysis in transfected A549 and SK-MES-1 NSCLC cells. n = 3 in (A–G). *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: We harvested protein extracts from collected tissue specimens (~50 mg) or cultivated cells (1 × 107) and conducted immunoblot analysis as described previously [18] with rabbit anti- NSUN4 polyclonal (#29786- 1- AP, 1–4000, Proteintech), rabbit antiCDC20 polyclonal (#10252- 1- AP, 1–8000, Proteintech), rabbit anti- SOX2 polyclonal (#11064- 1- AP, 1–600, Proteintech), rabbit anti- CD133 monoclonal (#ab222782, 1–2000, Abcam), rabbit anti- KLF4 polyclonal (#11880- 1- AP, 1–6000, Proteintech), or mouse anti- β- actin monoclonal (#66009- 1- Ig, 1–50 000, Proteintech).

Techniques: Inhibition, In Vitro, Expressing, Western Blot, Transfection, Flow Cytometry

Journal: Thoracic cancer

Article Title: NSUN4 Facilitates the Activity of Oncogenic Protein CDC20 to Promote NSCLC Development by Mediating m5C Modification of CDC20 mRNA.

doi: 10.1111/1759-7714.70023

Figure Lengend Snippet: FIGURE 5 | NSUN4 affects NSCLC cell malignant phenotypes through CDC20. (A–H) A549 and SK-MES-1 NSCLC cells were subjected to intro- duction with vector + sh-ctrl, NSUN4 expression construct + sh-ctrl, vector + sh-CDC20, or NSUN4 + sh-CDC20. (A) CDC20 protein expression by immunoblotting in cells transfected as indicated. (B) The number of formed colonies by colony formation assay with cells transfected as indicated. (C) Cell apoptotic ratio by flow cytometry with cells transfected as indicated. (D and E) Cell migratory rate and invasiveness by transwell assay with transfected cells. Scale bars: 100 μm. (F) Measurement of sphere formation with transfected NSCLC cells. (G and H) Expression of SOX2, CD133, and KLF4 proteins by immunoblot analysis in transfected A549 and SK-MES-1 NSCLC cells. n = 3 in (A–G). *p < 0.05, **p < 0.01, ***p < 0.001, ns: non-significant.

Article Snippet: We harvested protein extracts from collected tissue specimens (~50 mg) or cultivated cells (1 × 107) and conducted immunoblot analysis as described previously [18] with rabbit anti- NSUN4 polyclonal (#29786- 1- AP, 1–4000, Proteintech), rabbit antiCDC20 polyclonal (#10252- 1- AP, 1–8000, Proteintech), rabbit anti- SOX2 polyclonal (#11064- 1- AP, 1–600, Proteintech), rabbit anti- CD133 monoclonal (#ab222782, 1–2000, Abcam), rabbit anti- KLF4 polyclonal (#11880- 1- AP, 1–6000, Proteintech), or mouse anti- β- actin monoclonal (#66009- 1- Ig, 1–50 000, Proteintech).

Techniques: Plasmid Preparation, Expressing, Construct, Western Blot, Transfection, Colony Assay, Flow Cytometry, Transwell Assay

Journal: Thoracic cancer

Article Title: NSUN4 Facilitates the Activity of Oncogenic Protein CDC20 to Promote NSCLC Development by Mediating m5C Modification of CDC20 mRNA.

doi: 10.1111/1759-7714.70023

Figure Lengend Snippet: FIGURE 6 | NSUN4 depletion hinders the growth of A549 subcutaneous xenografts. (A–E) A549 subcutaneous xenografts were generated by implanting sh-ctrl or sh-NSUN4#2 lentivirus-infected A549 cells. After 30 days, xenografts were harvested. n = 5 for each group. (A) Growth curves of A549 subcutaneous xenografts (n = 3). (B) Representative pictures of A549 subcutaneous xenografts. (C) Tumor average weight was calculated (n = 3). (D) Expression of NSUN4, CDC20, SOX2, CD133, and KLF4 proteins by immunoblot analysis in A549 subcutaneous xenografts (n = 3). (E) Expression of NSUN4, CDC20, SOX2, CD133, and KLF4 proteins by immunohistochemistry in sections of subcutaneous xenografts. **p < 0.01, ***p < 0.001.

Article Snippet: We harvested protein extracts from collected tissue specimens (~50 mg) or cultivated cells (1 × 107) and conducted immunoblot analysis as described previously [18] with rabbit anti- NSUN4 polyclonal (#29786- 1- AP, 1–4000, Proteintech), rabbit antiCDC20 polyclonal (#10252- 1- AP, 1–8000, Proteintech), rabbit anti- SOX2 polyclonal (#11064- 1- AP, 1–600, Proteintech), rabbit anti- CD133 monoclonal (#ab222782, 1–2000, Abcam), rabbit anti- KLF4 polyclonal (#11880- 1- AP, 1–6000, Proteintech), or mouse anti- β- actin monoclonal (#66009- 1- Ig, 1–50 000, Proteintech).

Techniques: Generated, Infection, Expressing, Western Blot, Immunohistochemistry

Journal: Cell Reports Medicine

Article Title: Outer radial glia promotes white matter regeneration after neonatal brain injury

doi: 10.1016/j.xcrm.2025.101986

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Sox2 , Millipore , Cat# AB5603; RRID: AB_2286686.

Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Blocking Assay, Staining, Knock-Out, In Situ, Multiplex Assay, Sequencing, Software

Journal: Journal of respiratory biology and translational medicine

Article Title: Mitochondrial Lon Peptidase 1 Controls Diaphragm and Lung Development in a Context-Dependent Manner

doi: 10.70322/jrbtm.2025.10008

Figure Lengend Snippet: ( A – C ) Representative images of SOX2 and SOX9 staining in the lungs of Shhcre;Lonp1 and control mice at E13.5. Scale bars, 200 μm. Quantifications of SOX2 ( B ) and SOX9 ( C ) were shown, respectively. ( D , E ) In situ hybridization analysis of Sox2 ( D ) and Sox9 ( E ) RNAs in the lungs of Shhcre;Lonp1 and control mice at E12.5. Scale bars, 200 μm. Selected airway regions were boxed and magnified on the top right. ( F , G ) Representative images of aSMA staining in the lungs of Shhcre;Lonp1, and control mice at E13.5 ( F ). Selected airway regions were boxed and magnified on the right: scale bars, 200 μm. Quantifications were shown in ( G ).

Article Snippet: Primary antibodies with final concentrations used for immunofluorescence staining are: rabbit anti-SOX2 polyclonal antibody [8 mg/mL] (NB110–37235, Novus Biologicals, Centennial, CO, USA), mouse anti-SOX9 monoclonal antibody [5 mg/mL] (AMAB90795, Sigma, Tokyo, Japan), mouse anti-aSMA monoclonal antibody [5 mg/mL] (A2547, Sigma), mouse anti-E-cadherin monoclonal antibody [8 mg/mL] (610181, BD Transduction Laboratories, NJ, USA), rabbit anti-E-cadherin polyclonal antibody [5 mg/mL] (3195, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-FOXA1 monoclonal antibody [5 mg/mL] (ab173287, Abcam, Cambridge, UK) and rabbit anti-FOXA2 monoclonal antibody [5 mg/mL] (ab108422, Abcam).

Techniques: Staining, Control, In Situ Hybridization